The specific activity of an enzyme is another common unit. This is the activity of an enzyme per milligram of total protein (expressed in μmol min−1 mg−1). Specific activity gives a measurement of enzyme purity in the mixture.Click to see full answer. Similarly, you may ask, how do you calculate the specific activity of an enzyme?Therefore, specific activity is calculated by dividing the number of units/mL by the protein concentration in mg/mL to get μmol/min/mg. For example: The specific activity of the isolated enzyme was measured at 150 μmoles/min/mg protein before purification and 800 μmoles/min/mg, after purification.Subsequently, question is, what is the difference between enzyme activity and specific activity? The enzyme activity refers to the number of moles of product formed per unit time. Once we know the enzyme activity and the concentration of the enzyme, we can then determine the specific activity of the enzyme. The specific activity is the ratio of the enzyme activity to enzyme concentration. Hereof, what is the activity of an enzyme? Enzyme Activity. Enzyme activity is a measure of the catalytic ability and there are two methods to measure enzyme activity: one of them is to measure the decrease in substrate concentration in a period of time, and the other is to measure the increase in concentration of a product after a period of time.What is used to measure specific activity?Specific activity. Thus, specific activity is defined as the activity per quantity of atoms of a particular radionuclide. It is usually given in units of Bq/g, but another commonly used unit of activity is the curie (Ci) allowing the definition of specific activity in Ci/g.

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